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文献和实验Biotin labeling and hybridization of probe to chromosomes
20 X SSC 2 µg sheared salmon sperm DNA (10 mg/ml) 2 µl probe DNA
DNA Probe Purification Protocol
assembled in a clean 2 mL collection tube (provided) and centrifuge in a microcentrifuge at 10,000 x g for 1 min at room temperature. Discard the liquid. 4. Wash the column by adding 750 ul of Wash Buffer diluted with absolute ethanol. Centrifuge at 10
Microarray Temperature Optimization Using Hybridization Kinetics
In any microarray hybridization experiment, there are contributions at each probe spot due to the match and numerous mismatch target species (i.e., cross-hybridizations). One goal of temperature optimization is to minimize the contribution
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