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文献和实验RUVKUN Antibody Staining Protocol revised 10/29/90
above (total volume= 5ml). Then add heptane(or water) and MeOH; EGTA. 100mM Tris Cl pH 7.4 1% Triton X-100 1 mM EDTA 1M H3BO3 0.5 M NaOH 0.1% BSA 1X PBS 0.5% Triton X-100 0.05% Na Azide 1mM EDTA
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
°C 5. Sterile serological pipettes (5-mL, 10-mL) 6. Centrifuge 7. 15-mL centrifuge tubes 8. 60-mm and 100-mm tissue culture-treated dishes 9. 6-well tissue culture-treated plates 10. 25-gauge 1½-inch needle
transfer supernatant to a new 2 ml tube and add100 ul HTR Reagent. Mix throughly by vortexing for 10 seconds. 7. Incubate at room temperature for 2 minutes. Centrifuge at full speed (13,000 x g) for 2 minutes. 8. Transfer 500 ul
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