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文献和实验. They are open to modification and should not be construed as a comprehensive set of rules. Mouse Breeding Recommendations 1. Keep accurate breeding records. Make a pedigree for each transgenic founder or embryonic stem cell-mouse chimera. 2. Mate
any problems in knocking down the targeted genes. In order to design a siRNA duplex, we search for the 23-nt sequence motif AA(N19)TT (N, any nucleotide) and select hits with approx. 50% G/C-content (30% to 70% has also worked in our hands). If no suitable
3'-UTRs and have not experienced any problems in knocking down the targeted genes. In order to design a siRNA duplex, we search for the 23-nt sequence motif AA(N19)TT (N, any nucleotide) and select hits with approx. 50% G/C-content (30% to 70
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