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文献和实验Endothelial Cell Systems - Instructions For Use
: cells/ml = (n) x 104, where: n = the average cell count per square of the four corner squares counted. Example: If the calculated average (n) of cells in the four 1 mm corner squares of the hemacytometer is 30: cells/ml = (n) x 104 (or) cells/ml
100 ml, sterile filter to remove particulate matter pour into a bottle, and carefully mark the level of the meniscus Add DEPC to 0.1%, shake to dissolve DEPC Let stand overnight at 37o Autoclave 20 minutes After autoclaving
秀丽线虫RNA提取--C. elegans RNA prep
. 5. Pour the mixture into a 28 ml polycarbonate Oak Ridge tube. Spin in the 70Ti ultracentrifuge rotor for 20 minutes at 30K RPM at 20° to pellet debris (and any metallic stuff from the polytron). Pour off the supernatant into a clean 50 ml
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