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文献和实验Guanidine Hydrochloride Purification of Proteins From SDS
several dozen lock top eppendorf tubes.In the hood add approximately 0.5 ml Sigmacote to an eppendorf tube,invert several times and then pour the Sigmacote into the next tube etc...Remove the last traces of Sigmacote for each tube,rinse with Q,and autoclave
Guanidine Hydrochloride Purification of Proteins From SDS PAGE
add DTT and PMSF just before use Procedure • Sigmacote several dozen lock top eppendorf tubes.In the hood add approximately 0.5 ml Sigmacote to an eppendorf tube,invert several times and then pour the Sigmacote into the next tube etc...Remove
A safe method of extracting DNA from Coccidioides immitis
, Academic Press, San Diego). The pellet is resuspended in 100 ul TE, and 5 ul of this is electrophoresed in agarose to check the yield - usually there is an easily visible band of high molecular weight DNA (Figure 1). We have successfully amplified the DNA
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