IRIS TIE DOWN KIT/JA-12 (SINGL

Native Chromatin Preparation and Illumina/Solexa Library Construction

cell isolation kit) per 107 cells. 12. Mix well and incubate for 15 min at 4°C-8°C. 13. Wash cells with T cell isolation buffer by adding 10X-20X the final labeling volume. Centrifuge at 300g for 10 min. Pipette

Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos

Micromanipulators (2) (Narishige) Petri dishes (60 mm) (BD Falcon, 353002) Petri dishes (100 mm) (BD Falcon, 351029) Picoliter injector (Harvard Instruments, PLI-100) Platinum wires (3 x 0.35 mm and 80 x 0.3 mm) Scissors, angled iris (Fine

Differential cDNA Screening Procedures

. Much of the above can be supplied in kit forms by major suppliers, particularly: Stratagene, Promega and BRL. Their tech. service are normally pretty helpful.