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文献和实验Transformation of E. coli by Electroporation
ml of SOB (without magnesium) in a 1 liter flask. Grow for 2 to 3 hours with vigorous aeration at 37EC until the cells reach an OD550 = 0.8. 3. Harvest cells by centrifugation at 5000 RPM (2,600 g) in a GSA rotor for 10 min. (Make sure you use
! For preparative rotors, be sure the rotor cover is in place and properly screwed down, where appropriate. NEVER use a rotor without its lid, when one is supplied - the screw actually holds the rotor to the motor shaft. Check that the centrifuge chamber is clean
and properly screwed down, where appropriate. NEVER use a rotor without its lid, when one is supplied - the screw actually holds the rotor to the motor shaft. Check that the centrifuge chamber is clean, defrosted and that all membranes
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