Beaker, 400 mL, Enhanced Perfo

Metal-Enhanced Immunoassays

was performed in a glass 100-ml beaker as follows. At intensive stirring, 20 drops of NaOH solution (5 M) were added to AgNO3 solution (500 mg) in Milli-Q water (60 ml), and dark brown precipitate formed immediately. Approximately 2 ml of 30% NH4 OH solution

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

-tetracycline (cTc) was used to inactivate the Tet repressor. The inducer cTc was suspended in LB at a concentration of 400 μg/ml and autoclaved in a pressure cooker for 20 min, then stored in dark environment. Induction of I-SceI was achieved by adding cTc

细胞密度梯度分离介质Ficoll：人外周血单个核细胞的分离

Ficoll：一种已灭菌的，特定密度的密度梯度离心介质。被广泛用作从外周血、骨髓及脐带血中分离高活性的单个核细胞。 Ficoll-Paque溶液包含： Ficoll PM400：由高分子量蔗糖（Mr 400K）聚合物与表氯醇聚合合成(凝集红细胞) 泛影酸钠：使溶液在低粘度下具有高密度 EDTA二钠钙 Ficoll的保存：开盖前保存在4℃~30℃（避光），开盖后保存在 4℃~8℃（避光） Ficoll 产品细分如下： 使用Ficoll (1.077g/ml) 分离