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文献和实验Purification of Plasmid from 50 ml-culture
of cold 70% ethanol. Discard the fluid. 14. Add to the pellet 400 ul of TE containing DNase-free RNase A (20 ug/ml). Incubate the tube for 30 min at 37 C. 15. After 30 min, carefully check the content of the tube. If nucleic acid pellet
ES CELL DNA EXTRACTION: TUBE ME
in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM Tris-HCl pH7.5 - 0.5 ml of 2M 10 mM EDTA - 2 ml of 0.5M 10 mM NaCl - 0.2 ml
E. Z. N.A.TM X-press Plasmid Protocol
at maximum speed (13,000 x g) for 1 minute to dry the column. 14. Transfer the X-press spin column into a new 1.5 ml centrifuge tube. Add 50 uL Elution Buffer (10mM Tris Hcl pH 8.5) directly onto the center of the membrane. 15. Centrifuge
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