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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
Goat Anti-Rabbit IgG (H L) Antibody 实验设备 1. Sterile cell culture hood (i.e., biosafety cabinet) equipped with a stereo microscope 2. Inverted microscope 3. Incubator set at 37°C, 5% CO2 4. Water bath set at 37
之mycoplasma (e.g. M. hyorhinis)。不需培养mycoplasma作为正反应对照组,避免可能之污染。 缺点︰PCR反应很灵敏,易有伪阳性结果。此方法尚在评估中,故结果仅作为参考和内部品管之一部份。 材料与设备: ATCC mycoplasma detection kit:可作50~100 reactions. 提供1st stage primer mixture与2nd stage primer mixture positive control DNA (A. laidlawii
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
II. REFERENCES •Transfectol-LS Mammalian Transient transfection Kit. GeneChoice; TR10v2 KT-011106 III. EQUIPMENT, MATERIALS, AND REAGENTS • CO2 incubator, Forma Scientific incubator • BioSafety Cabinet, • Orbital Shaker, Forma
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