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- Beckman Coulter贝克曼库尔特
- 美国
- FG7025100
- 2025年07月11日
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文献和实验Modified Eberwine (antisense) RNA Amplification Protocol
not to spill. Mix by pipeting 5-10X, then transfer slurry to Phase lock gel tubes. Spin 5 minutes maximum speed (15k x g) at room temperature. Transfer aqueous phase to RNase free 1.5 ml eppendorf tube. Add 70 uL 7.5M ammonium acetate (in DEPC water
哺乳动物RNAi技术 Mammalian RNA Interference
at 3000 rpm (700g) for 4 minutes at 4ºC. Resuspend the cell pellet in ice-cold PBS and centrifuge again. Remove the supernatant and add 25 µl of 90ºC 2x concentrated Laemmli SDS sample buffer to the cell pellet obtained from one well of a 24
Endothelial Cell Systems - Instructions For Use
Heparin 10 ml FBS (Fetal Bovine Serum) 2% EGM-2, 25 mls in EGM-2-MV 5% 0.5 ml Gentamicin, Amphotercin 4. ReagentPack™ (CC-5034) contains one 100 ml bottle of each of the following subculture reagents: HEPES Buffered Saline Solution (HEPES-BSS) (CC
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