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文献和实验Purification of Plasmid from 50 ml-culture
4. Resuspend the pellet in 5 ml of Solution I. 5. Add 10 ml of Solution II. Mix well by inverting the bottle more than 10 times. The solution should be clear after mixing Solution II. 6. Add 7.5 ml of Solution III. Mix well as above. 7. Spin
ES CELL DNA EXTRACTION: TUBE ME
in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM Tris-HCl pH7.5 - 0.5 ml of 2M 10 mM EDTA - 2 ml of 0.5M 10 mM NaCl - 0.2 ml
, a pair of frogs were mated (see Grainger Lab X. tropicalis Mating Protocol for details) and the resultant tadpoles were used in the following karyotyping protocol. 2. Place 10 Nieuwkoop & Faber Stage 26-34 tadpoles into a dish of deionized, distilled
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