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文献和实验at 750 ng/ul for RLGS analysis in this plant. Ascertain the size of obtained DNA fragments are larger than lamda DNA using 0.5 % agarose gel electrophoresis (100 - 200 kb recommended). B. Preparation of gel holder Cut teflon-tubing (NICHIAS AWG
than lamda DNA using 0.5 % agarose gel electrophoresis (100 - 200 kb recommended). B. Preparation of gel holder Cut teflon-tubing (NICHIAS AWG-11, inner diameter: 2.4 mm) into 65 cm (cut a top at an angle of about 20 degrees
Fluorescein labeling of proteins
as preservative. Determine concentration and F/P ratio by measuring absorbance at 280 nm and at 495 nm.For antibodies,use the following formulae to get approximate values: Protein concentration (mg/ml)= (OD280 - 0.35 x OD495)/ 1.4 F/P ratio = (3.3 x OD495)/ (OD
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