- ￥2552
- Beckman Coulter贝克曼库尔特
- 美国
- A35473
- 2025年07月07日
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文献和实验Bromodeoxyuridine Immunohistochemistry
endogenous peroxidases in a staining dish. 5. 1XPBS, 4 X 5 min., RT 6. Pepsin/HCI, 20 min. (try 30 - 40 min.), RT in a humidifies chamber. Prepare 200 mg/ml pepsin, 0.01N HCI in PBS [from 10 mg/ml pepsin prepared fresh in ddH2O(50x) and 2N HCI (200
70% EtOH to 1/2 fill eppendorf. Vortex 5sec. Spin 10 min at 1350 rpm. Discard EtOH Quick spin. Remove excess remaining EtOH using P200 pipette. Air Dry 10 min Add 20l 1X loading buffer (Stock 6X
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
. For cytospin slides : i) fix air dried cell samples with a freshly prepared paraformaldeyde solution (4% in PBS, pH 7.4) for 30 min at room temperature; ii) rinse slides with PBS and incubate in permeabilisation solution (0.1% Triton X-100, 0.1% sodium citrate
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