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文献和实验UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
. If necessary, adjust the seal steam pressure by turning the seal steam knob (red). This should normally be open ~ 1 1/4 turn (black mark on knob should be ~straight up). The seal steam condenser water knob should be fully open. See Steve Hahn
Transformation of E. coli by Electroporation
-DNA mixture between the bosses in a Micro-Electroporation Chamber. Do not leave an air bubble in the droplet of cells; the pressure of a bubble may cause arcing and loss of the sample. Place the chamber in a slot in the Chamber Rack and note
puller without touching your fingers to the part that will be heated, or touching the filament to the heating elements. Tighten the filament in with the top knob, DON'T EVER TOUCH THE MIDDLE KNOB!!!! Slide the bottom unit up all the way, then tighten
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