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文献和实验Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels
2-Shake gently at room temperature approximately 15 min. 3-Aspirate off the EC lysis solution (be careful not to suck up the agarose plug!). 4-Add 1 ml per well EC lysis solution with lysozyme and RNase. 5-Seal plate inside a heat-sealable bag
). Pour the running gel to about 1 cm below the wells of the comb (~20 ml). Seal with 1 ml water-saturated 1-butanol. (Can stop here and leave gel as is overnight if you want.) When gel has set, pour off the butanol
Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels
3 -Aspirate off the EC lysis solution (be careful not to suck up the agarose plug!). 4 -Add 1 ml per well EC lysis solution with lysozyme and RNase. 5 -Seal plate inside a heat-sealable bag. 6 -Place bag (submerged
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