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文献和实验Construction and Manipulation of Large-Insert Bacterial Clone Libraries
libraries equivalent to 6 � 8 x haploid genomes would be sufficient for different genome research purposes, including global genome physical mapping and sequencing. Large-insert, ordered BAC and BIBAC libraries
Analysis of Proteins using Small Format 2D Gel Electrophoresis
twice with PBS-A and lysed in 0.2 ml of 2D lysis buffer (0.01M Tris-HCl, pH 7.4, 1mM EDTA, 8M urea, 0.05M dithiothreitol, 10% (v/v) glycerol, 5% (v/v) Nonidet P-40, 200µg/ml RNAse A, 6% (w/v) pH 3.5-10 carrier ampholytes ("Resolyte", Merck
Using Soil DNA Kit (D5625) to Clean Up DNA containing inhibitors
collection tube in next step. 12. Discard liquid and re-insert the column to the empty collection tube, centrifuge the column at 14,000 x g for 2 min at room temperature. This step is critical in removing traces of ethanol that will interfere
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