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文献和实验. The covalent addition of a methyl group to cytosine bases in the CpG dinucleotide sequence holds particular analytical advantages. Working with DNA as an analyte molecule is robust and samples are unproblematic to collect and handle. Also changes in DNA
tag as a purification handle. Provided that the isolated protein formed UV-induced crosslinks with the RNA component of the studied RNP, the isolation of the protein resulted in the co-isolation of the covalently bound RNP RNA. The isolated protein
DNA: DNA in situ hybridization
oligonucleotides (14-30bp long) are being used increasingly as probes, when salt and temperature conditions need to be adjusted. Oligonucleotides are also used for in situ primer extension and in situ PCR protocols, particularly to detect repetitive
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