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- Beckman Coulter贝克曼库尔特
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- 2025年07月14日
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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
1 Polarizer Rotation Mount Newport RM25A 1 ?" spacer for 1" diameter post
is then placed in the humidified chamber which is incubated at room temp. Wash in PBS + 1% NGS 3 X 10 min. Incubate in secondary antibody (FITC or Texas Red conjugated) at a dilution of 1:50 for 1 hour in a humidified chamber at room temp.
免疫荧光技术--Immunofluroescence Technique
is inverted onto a glass slide. The slide is then placed in the humidified chamber which is incubated at room temp. Wash in PBS + 1% NGS 3 X 10 min. Incubate in secondary antibody (FITC or Texas Red conjugated) at a dilution of 1:50 for 1 hour
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