Tubing, Tygon 1/4 ID x 3/8 O

交联染色质免疫共沉淀（X-ChIP）实验设计

冻存于-20°C.样品采用蛋白酶K处理可使相邻肽段剪切成羧基团的芳香族氨基酸和脂肪族氨基酸。蛋白质和DNA之间的交联通过DNA的纯化被打断。3检测DNA浓度，取5ul纯化的DNA到995ulTE中得到一个原溶液的200倍稀释测其OD260。那么DNA的浓度就为OD260x10,000.μg/ml。这样就可计算出染色质制品的DNA浓度。4 免疫共沉淀1 从2.2得来的染色质制品，推荐每个IP样品都采用25ug的DNA的量。每个样品按1：10的量加入RIPA溶液。需要一个样本作为空珠子的对照。2在除空

E. Z. N.A.TM X-press Plasmid Protocol

实验试剂   Materials to Be Provided by User 1. Ice 2. Isopropanol (100%) 实验设备   Materials to Be Provided by User 1. Tabletop micro-centrifuge capable of 13,000 x g 2. Nuclease Free

E-Z 96 X-press Plasmid Vacuum Protocol

selective antibiotic. Incubate 14-16 hours at 37℃ 600 with vigorous shaking until an OD of 2.0-4.0 is achieved. 2. Pellet 1-2 mL bacterial culture in a 2.0 ml 96-well deep-well plate by centrifugation at 3000 x g for 15 min at room temperature.