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文献和实验the chromosomal DNA). 4. Add a total of 140 ml of alkaline lysis solution (70 ml for each bottle), gently mix, and incubate for 5 minutes in an ice-water bath. 5. Add 105 ml of 3M NaOAc, pH 4.8 (52.5 ml for each bottle), cap tightly, gently mix
the chromosomal DNA). 4. Add a total of 140 ml of alkaline lysis solution (70 ml for each bottle), gently mix, and incubate for 5 minutes in an ice-water bath. 5. Add 105 ml of 3M NaOAc, pH 4.8 (52.5 ml for each bottle), cap tightly, gently mix
for an additional 30 minutes. 5) Remove the plate from the water bath and gently agitate the plate by swirling or placing the plate on a shaker set to low speeds until the bead pellet is completely dispersed. Note: If the pellet is not completely
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