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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见瓶身
- 保质期:
/
- 英文名:
/
- 库存:
999
- 供应商:
麦飞生物
- CAS号:
/
- 规格:
5 ml
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文献和实验bath for 3 min. 8.Add 100 µl 2x Laemmli sample buffer(or use 75μl of 3X Laemmli sample buffer). 9.Place in boiling water bath for 1 min. 10.Spin briefly in Tomy (at room temp--15 sec,3K),remove liquid with a ml pippetteman tip,and transfer to 1.5 ml
Preparation of protein extracts for western blot
.Remove supernatant and transfer to new tube.This can be used immediately or frozen at -20℃.If frozen,boil sample for 3 min.prior to loading on gel. 12.Load about 10-20 µl per minigel lane,depending on initial OD.For a preparative gel,load 150μl
), capable of delivering buffer at a flow rate of 0.152 ± 0.002 ml/min over long time periods (see Critical Paramameters ) Teflon syringe‐loading sample injector (Rheodyne model 7125
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