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文献和实验Replication timing by density transfer
Replication timing by density transfer M. K. Raghuraman 1. Grow cells at least 7 generations in 0.1% (w/v) [13
Research) or a Perkin Elmer Cetus cycler was used. The amplification products were separated by gel electrophoresis (3 V/cm) through a 1.6% gel (0.8% agarose and 0.8% Synergel "(Diversified BioTech, Newton Centre, CT))(9) in recirculating TAE " buffer.
RACE System for Rapid Amplification of cDNA Ends
of the wash buffer concentrate into a 50-ml graduated cylinder. 2) Add 18 ml of distilled water and 21 ml of absolute ethanol. Mix thoroughly. 3) Transfer to an appropriate-sized glass bottle. Cap and store at 4°C. 2. 70% Ethanol Wash for S
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