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文献和实验Sperm DNA Damage Measured by Comet Assay
electrophoresis is a relatively simple and sensitive method for measuring strand breaks in DNA in individual sperm. During this procedure, sperm cells are embedded in a thin layer of agarose on a microscope slide and lysed with detergent under high salt conditions
and DNA sequences. Over the last few years, the use of PNAs has proven its efficacy in cytogenetics for the rapid in situ identification of human chromosomes. Multicolour PNA–FISH protocols have been described and their adaptation to human spermatozoa
Standard Protocol For Isolation of DNA From Dried Blood, Body Fluids and Sperm
away. High quality DNA is finally eluted in sterile deionized water or low salt buffer. Each HiBind® column can bind approximately 100 ug DNA. Use of more than 30 mg tissue or 107 cells is not recommended. 实验步骤 Dried blood, body
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