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- 文献和实验
- 技术资料
- 保存条件：
见瓶身
- 保质期：
/
- 英文名：
/
- 库存：
999
- 供应商：
麦格生物
- CAS号：
9002-92-0
- 规格：
1 kg
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文献和实验HIGH RESOLUTION GENETIC FOOTPRINTING
This web site was designed to provide detailed protocols for genetic footprinting, a technique for high-resolution mapping of the functional organization of cloned genes (Singh, Crowley and Brown (1997) PNAS 94: 1304-1309
Purification of Endotoxin Free mAbs
purity is required. Ion exchange, rather than affinity purification (eg protein A or G) is recommended, because ion exchange materials are high capacity, cheap enough to be disposable, and can easily be sanitized with 0.5M NaOH. The following method
Replication timing by density transfer
(frozen slanted to maximize the surface area). During the experiment, mix 20 ml of cells with 1/50 volume of 10% Na-azide in a 35 ml Corex tube (on ice) and immediately transfer the mix to a tube of frozen EDTA/azide. Alternatively, squirt the 10% azide
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