Recombinant full-length human GFAP isotype 1, expressed in and purified from E. coli.
亚型:
IgG
保存条件:
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Horse, Bovine, Porcine, Rat, Mouse
宿主:
Rabbit
应用范围:
WB, ICC/IF, IHC, IP
靶点:
GFAP
抗体英文名:
Anti-GFAP Antibody - Identical to Abcam (ab7260) and Novus (NB300-141) (A85419)
规格:
50µl/100µl
规格:
50µl
产品价格:
¥5530.0
规格:
100µl
产品价格:
¥7160.0
Rabbit polyclonal antibody to GFAP for WB, ICC/IF, IHC and IP with samples derived from Human, Horse, Bovine, Porcine, Rat and Mouse.
Figure 1: Immunofluorescence - Anti-GFAP Antibody (A85419) Mixed neuron-glial cultures stained with Anti-GFAP Antibody (red) and Anti-Vimentin Antibody (A85421 | green). The fibroblastic cells contain only vimentin and so are green, while astrocytes contain either vimentin and GFAP, so appear golden, or predominantly GFAP, in which case they appear red. Blue is nuclear DNA stain.
Figure 2: Immunofluorescence - Anti-GFAP Antibody (A85419) Immunofluorescent analysis of a rat cerebellum section stained with Anti-GFAP Antibody (A85419), at a dilution of 1:5,000, in green, and co-stained with Anti-MeCP2 Antibody (A104324), at a dilution of 1:500, in red. The nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion of the rat with 4% PFA, the brain was post-fixed for 1 hour, cut to 45 µm, and free-floating sections were stained with the above antibodies. The Anti-GFAP Antibody (A85419) stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The Anti-MeCP2 Antibody (A104324) specifically labels nuclei of certain neurons.
Figure 3: Western Blot - Anti-GFAP Antibody (A85419) Western blot analysis of different tissue lysates using Anti-GFAP Antibody (A85419), at a dilution of 1:5,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at about 50 kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.
Figure 4: Western Blot - Anti-GFAP Antibody (A85419) Western blot analysis of Anti-GFAP Antibody. Blot of rat brain lysate was probed with Anti-GFAP Antibody (1:5,000). A prominent band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to rodent GFAP.
Grochow, Thomas et al. “Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondii.” Communications biology vol. 6,1 1209. 27 Nov. 2023, doi:10.1038/s42003-023-05576-6 Mustapha, Oluwaseun et al. “Neocortex neurogenesis and maturation in the African greater cane rat.” Neural development vol. 18,1 7. 13 Oct. 2023, doi:10.1186/s13064-023-00175-x Kalusa, Mirjam et al. “Developmental Differences in Neocortex Neurogenesis and Maturation Between the Altricial Dwarf Rabbit and Precocial Guinea Pig.” Frontiers in neuroanatomy vol. 15 678385. 31 May. 2021, doi:10.3389/fnana.2021.678385