In Vitro: Preparation of Green DND-26 working solution 1. Preparation of the stock solution 1.1 Restore the solution to the room temperature and concentrate it to the bottom of the tube by instantaneous centrifugation. 1.2 Dilute 1 mM storage solution to the working solution using medium or appropriate buffer (such as PBS) . The recommended working solution is 50-100 nM. Note: Please adjust the concentration of Green DND-26 working solution according to the actual situation, and use it now. Cell staining 2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. 2.2 Add 1 mL of Green DND-26 working solution, and then incubate at room temperature for 30 minutes. 2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. 2.4 Wash twice with PBS, 5 minutes each time. 2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer. Storage -20°C save, Protect from light