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PAA4
MCE 国际站：PAA4
产品活性：PAA4 是一种以甲基化物碳为中心的多核 Au(I) 簇。PAA4 显示出抗增殖活性。PAA4 以时间依赖性方式增加 pH2AX 的表达。PAA4 在原位膀胱癌小鼠模型中显示出抗肿瘤作用。
研究领域：Others
作用靶点：Others
In Vitro: PAA4 (0-4 µM; 24 h) shows no significant cytotoxicity in EJ, HUVEC, SV-HUC-1 cell lines with IC50 values of 2.1, 0.80, 2.3 µM, respectively.
PAA4 (4 µM; 1, 3, 6 h) causes significant DNA damage as indicated by the increase of histone H2AX phosphorylation (pH2AX).
In Vivo: PAA4 (1.5 µM, 100 µL; intravesical delivered into the bladder; 5 times every other day for 8 days) shows anti-tumor activity in mouse.
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文献和实验Phosphoamino acid analysis:Mark Kamps's method
more than 3 µl is tedious,so don't use much H20.On the other hand,I wouldn't use much less than 6 µl so as to have good recovery. 7.Spot 1 µl PAA standards (about 0.3 µg each of PSer,PThr,and PTyr)on a cellulose thin layer chromatography plate and then spot
Phosphoamino acid analysis:Mark Kamps method
on Speedivac. (It takes about 3 hr.) 6. Resuspend in H2O, and microfuge 5 min. Spotting more than 3 µl is tedious, so don't use much H20. On the other hand, I wouldn't use much less than 6 µl so as to have good recovery. 7. Spot 1 µl PAA
Synthesis of Drug/Dye-Incorporated PolymerProtein Hybrids
We present here a general methodology for significantly increasing the number of dye/drug molecules that can be attached per protein molecule. As a demonstration of this approach, poly(acrylic acid) (PAA)-based near-infrared fluorescence
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