In Vitro: TH10785 (6.25 μM, 30 min) induces a de novo β, δ-elimination in vitro, allowing for AP sites as new substrates. TH10785 (10 μM, 0-2 min) allows OGG1 to increase DNA repair by addressing AP sites. TH10785 (0-20 μM, 72 h) induces OGG1 β, δ-lyase activity shifts cells toward PNKP1 dependence. TH10785 (2 μM) has affinity to OGG1 (KD=5.5 μM) increased when adding an AP site analog containing double-stranded DNA (KD=1.3 μM).