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- 文献和实验
- 技术资料
- 保存条件:
4°C, sealed storage, away from moisture
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
795316-15-3
- 规格:
10 mM * 1 mL/1 mg/5 mg/10 mg/25 mg/50 mg
| 规格: | 10 mM * 1 mL | 产品价格: | ¥1465.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥370.0 |
| 规格: | 5 mg | 产品价格: | ¥940.0 |
| 规格: | 10 mg | 产品价格: | ¥1500.0 |
| 规格: | 25 mg | 产品价格: | ¥2950.0 |
| 规格: | 50 mg | 产品价格: | ¥4700.0 |
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ACG416B
CAS No. : 795316-15-3
MCE 国际站:ACG416B
产品活性:ACG416B (compound 18) 是一种有效的胆碱激酶 (ChoK) 抑制剂,IC50 值为 0.4 μM。ACG416B 对 HT-29 人结肠癌细胞具有更高的 ChoK 抑制和抗增殖活性。
研究领域:Others
作用靶点:Others
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文献和实验图2 APC 5'末端的扩增。用对应APC mRNA的位置5846的GSP反转录1mg Hela总RNA得到APC mRNA。cDNA被纯化、加尾,和用对应位置1797(A带)和位置1632(B带)的引物和AAP一起,用ELONGASE酶混合物来扩增(94℃ 30s,接着35个循环的94℃ 15 s,55℃ 30 s,68℃ 5 min)。初步PCR用TE缓冲液稀释100倍,取1ml用引物APC-1632(A带)或引物APC-1242(B带)和AUAP分别重新扩增(30个循环),每个PCR
in the thermacycler at: a. 44°C for 1 hr. b. 92°C for 10 min to inactivate the reverse transcriptase. 4) Store reaction at �20°C or proceed to the PCR . Step Two: PCR A) Primer Preparation 1) Primers are shipped in dry form. Briefly spin the tube
N u c le u s , vol. 5, C h r o m a tin , part B. (Busch, H., ed.), Academic Press, New York, pp. 416-468. 9. Bourbon, H. M., Bugler, B., Caizergues-Ferrer, M., and Amalric, F. (1983) Roleof phosphorylation on the maturation pathways
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