In Vitro: Anti-inflammatory agent 64 (2 μM, 4 h) can significantly protect cells and promote cell proliferation in RAW264.7 cells stimulated by H2O2 (0.4 mM. Anti-inflammatory agent 64 (2 μM, 24 h) inhibits the production of NO (IC50=0.9 μM) in RAW264.7 cells stimulated by LPS (1 μg/mL) and has anti-inflammatory effect. Anti-inflammatory agent 64 (0.125-2 μM, 4-24 h) significantly reduces the protein level of ROS and enhances HO-1 protein level in RAW264.7 cells stimulated by Rot (5 μM) and LPS (1 μM/mL) and has antioxidant effect. Anti-inflammatory agent 64 (0.5-2 μM, 24 h) can restrain IL–6 in dose dependent manner (IC50=1.73 μM) and TNF-α (IC50=1.52 μM). Anti-inflammatory agent 64 (0.5-2 μM) can inhibit the expresstion of iNOS protein. Anti-inflammatory agent 64 (0.5-2 μM) inhibits the avtivition of NF-κB pathway by inhibiting the degradation and phosphorylation of IκBα in RAW264.7 cells stimulated by LPS (1 μg/mL).
In Vivo: Anti-inflammatory agent 64 (1-30 mg/kg, i.p., i.g., single dose) has anti-inflammatory and antioxidant activity in carrageenan (Cg) (HY-125474) induced paw edema model .