MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务。
CAS No. : 9014-74-8
MCE 国际站:Recombinant enterokinase
产品活性:Recombinant enterokinase (rEK) 是一种丝氨酸蛋白酶,具有胰蛋白酶原的生理激活剂的作用。Recombinant enterokinase 起着将胰蛋白酶原转化为其活性形式胰蛋白酶的作用。
研究领域:Metabolic Enzyme/Protease
作用靶点:Ser/Thr Protease
In Vitro: Protocol
1) Dissolution: Recombinant enterokinase can be diluted with 25 mM Tris-HCl pH 8.0 to a solution containing 0.1 U per 1 μL;
2) Enzyme digestion: During enzyme digestion, the reaction system contains 0.05-0.1 mg of fusion protein (protein concentration: 0.1-1 mg/mL) and 0.1-0.2 U of recombinant enterokinase. Enzyme digestion at 25℃ overnight (Note: complete Enzyme digestion takes 16-24 hours).
Notes
1) Temperature: It is not recommended to perform enzyme digestion at 37℃ as non-specific enzyme digestion may occur.
2) Reaction condition: Under the conditions of >200 mM imidazole, or >200 mM NaCl, or >5% glycerol, the enzyme digestion effect will be affected. You can refer to the following recommended methods for enzyme digestion:
*Recommended method: To obtain ideal enzyme digestion results, please dialyze the sample into 25 mM Tris-HCl 8.0 buffer and then perform enzyme digestion. If dialysis is inconvenient, the sample can be diluted until the imidazole content is below 100 mM, the NaCl concentration is below 50 mM, and the glycerol concentration is below 5% for enzyme digestion. The ratio of enzyme dosage to protein remains unchanged (i.e. 1U enzyme digests 500 μg protein).
3) Effect of phosphate: Phosphate has a strong inhibitory effect on Enterokinase, and phosphate cannot exist in the enzyme digestion system.
4) Removal of enterokinase: This product is a recombinant enterokinase with high enzyme activity. If the cleavage protein is used in small amounts, it does not need to be removed. If you need to remove recombinant enterokinase later, you can use anion exchange resin (eg. DEAE-FF) to elute it. Recommended elution conditions are as follows: Equilibrium Buffer: 25 mM Tris-HCl pH 8.0, Elution buffer: 25 mM Tris-HCl pH 8.0 with 100 mM NaCl.
5) Effect: If the protease-cutting effect is not satisfactory, the amount of enzyme can be increased appropriately or the enzyme-cutting time can be appropriately extended.
相关产品:Leupeptin hemisulfate | Aprotinin | AEBSF hydrochloride | Camostat mesylate | WNK463 | Proteinase K | Bradykinin | Nafamostat mesylate | PF-06446846 hydrochloride | Trypsin | Inclisiran | Benzamidine hydrochloride | WNK-IN-11 | Evolocumab | Chymotrypsin | Trypsin Inhibitor, soybean | SBC-115076 | Pep2-8 | 7-Amino-4-methylcoumarin | IPR-803 | Avoralstat | Alirocumab | Bococizumab | R-IMPP | Berotralstat dihydrochloride | UKI-1 | UK122 | PCSK9-IN-10 | PCSK9-IN-12
热门产品线:重组蛋白 | 药物筛选 | 天然产物 | 荧光染料 | PROTAC | 同位素标记物
Trending products:Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Dye Reagents | PROTAC | Isotope-Labeled Compounds