Gypsogenin 3-O-glucuronide,105

16HBE14o－细胞株的传代

此种细胞的培基最好用是DMEM！以前我们用1640，但生长及其缓慢。正常情况下，2天换一次液,4-6天传代一次.传代过程：1、直接倒掉DMEM培基2、pbs将细胞洗2次3、倒掉pbs4、加入0.25％胰酶（0.02%EDTA）1－2ml/培养瓶5、水平放置培养瓶，消化3－5分钟6、显微镜下观察：细胞形态变圆，而且细胞间界限明显后，或有极少数细胞漂浮起来就可终止消化。7、加入完全培基/血清中止消化（我的做法是：不倒掉胰酶）8、吹打：动作要快。按一定的顺序进行吹打，尤其是边缘和角落往往是16

Proteolytic Labeling With 18O for Comparative Proteomics Studies: Preparation of 18O-Labeled Peptides and the 18O/16O Peptide Mixture

of catalytic enzyme immobilized on beads facilitates its removal and termination of the exchange. In differential proteomic studies, heavy isotope-labeled peptides are combined with peptides carrying 16 O for isotope ratio measurements by mass

In Vitro Assessment of the Reactivity of Acyl Glucuronides

glucuronides are prepared with UDPGA-fortified microsomes and 1-O-β acyl glucuronide is established based on its sensitivity to hydrolysis by β-glucuronidase. The chemical degradation of 1-O-β isomer in 0.1 M phosphate buffer at pH 7.4 is monitored over time