货号：431731 Corning 储液瓶，125ml，

类器官培养与应用——胰岛类器官

- - - - - C687 KGF - - - - - 50ng/ml 50ng/ml - - - CH73 Vitamin C - - - - - - 0.25mM 0.25 mM 0.25 mM 0.25 mM   LDN-193189 - - - - - - 0.1μM 0.1 μM 0.1 μM -    GDC

Preparation of Tonsil Lysate

the day of experiment.Dissolve in 100% EtOH) 1 mM Benzamidine 5 mM Iodoacetamide Whatman Paper Centrifuge Bottles Funnel Squeeze bottle Several Beakers Homogenizer (Fisher PowerGen 125) 50ml Corning tubes Ice Bucket & Ice Dounce

v468 Chapter 2 Wnt活性蛋白分离及应用

(Cibacron Blue F3G-A coupledto Sepharose), ~100 mL packed into an empty column with100–200 mL of bed volume. 3. Binding Buffer: 1% (w/v) CHAPS, 150 mM KCl, and 20 mMTris-HCl, pH 7.5, sterile filtered. 4. Elution Buffer: 1% (w/v) CHAPS, 1.5 M KCl