- 询价
- Agrisera
- Sweden
- AS20 4402
- 2026年01月10日
- ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
- Rabbit
- Arabidopsis thaliana
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant, His6 tagged VPS35b of Arabidopsis thaliana UniProt: F4I0P8, TAIR: At1g75850
- 形态：
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
- 保存条件：
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- 克隆性：
Polyclonal
- 适应物种：
Arabidopsis thaliana
- 供应商：
Agrisera
- 宿主：
Rabbit
- 应用范围：
ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
- 靶点：
Anti-VPS35 | Vacuolar protein sorting-associated protein 35B (marker of PVC)
- 抗体英文名：
Anti-VPS35 | Vacuolar protein sorting-associated protein 35B (marker of PVC)
- 抗体名：
Anti-VPS35 | Vacuolar protein sorting-associated protein 35B (marker of PVC)
- 规格：
200 µg
- PRODUCT INFO
-
Immunogen: Recombinant, His6 tagged VPS35b of Arabidopsis thaliana UniProt: F4I0P8, TAIR: At1g75850 Host: Rabbit Clonality: Polyclonal Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized. Format: Liquid at 2 mg/ml. Quantity: 200 µg Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB) Recommended dilution: assay dependent (ELISA), 1: 400 (IF), 1: 100 (IP), 1: 1000 (WB) Expected | apparent MW: 89 | 98 kDa
 Arabidopsis thaliana 19 day-old seedlings were extracted to a crude extract and separated on 12.5 % SDS-PAGE and blotted to PVDF membrane in wet system. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.  Immunofluorescent localisation of VPS35. Tobacco NY-2 cells were transnformed with Arabidopsis thaliana VPS35 (left panel), PEP12 (middle panel, which is a PVC marker) Method described in details in: Yamazaki et al. (2008). |
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