Description :Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activatingfactor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently,MCP-1 has also been found to regulate adhesion molecule expression and cytokine productionin monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reactswith mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of naturalor recombinant MCP-1.
Preparation :The antibody was purified by affinity chromatography, and conjugated with PE under optimalconditions.
Concentration :0.2 mg/m
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :ICFC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by intracellular immunofluorescent staining withflow cytometric analysis. For immunofluorescent staining by flow cytometry, the suggested use ofthis product is ≤ 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent betitrated for optimal performance for each application.