Name :Zymoprep Yeast Plasmid Miniprep I
Cat # :D2001
Size :100 Preps
Brand :Zymo Research
_d2001_zymoprep_yeast_plasmid_miniprep_i.pdf
HIGHLIGHTS
Simple: Quickly and easily rescue plasmid from yeast.
Efficient Isolation: Works well with low-copy and hard to isolate plasmids.
High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR,
transformation, hybridization, etc.
DESCRIPTION
The Zymoprep Yeast Plasmid Miniprep I is a simple and efficient yeast plasmid miniprep that is
based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides
all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and
any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and
efficient, and there is no need for glass beads or phenol. Reliably recover plasmid DNA from yeast
colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and
hard to isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR,
and Southern blot analysis.
TECHNICAL SRECIFICATIONS
Applicable For
Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization
and other sensitive applications
Elution Volume
≥ 35 µl
Equipment
Microcentrifuge & Heat Block/Bath
Processing Volume
≤ 1.5 ml of Culture
Sample Source
Cell Culture (Colonies/Patches or Liquid Culture)
Size Range
No Size Limit
Yield
Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures