Zyppy Plasmid Miniprep Kit
Cat # Name Size
D4019 Zyppy Plasmid Miniprep Kit 100 Preps
D4020 Zyppy Plasmid Miniprep Kit 400 Preps
D4036 Zyppy Plasmid Miniprep Kit 50 Preps
D4037 Zyppy Plasmid Miniprep Kit 800 Preps
Brand :Zymo Research
_r1011_zymoclean_gel_rna_recovery_kit.pdf
HIGHLIGHTS
Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
High Quality: Ready for PCR, sequencing, cloning, and transfection.
DESCRIPTION
The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis
system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid
preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial
culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented
colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization.
The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA
from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in
transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR,
transcription, sequencing, and other sensitive downstream applications.
TECHNICAL SRECIFICATIONS
Applicable For
Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive
applications requiring pure DNA.
Elution Volume
≥ 30 µl
Equipment
Microcentrifuge and/or a vacuum manifold.
Processing Time
8 min
Purity
Typical Abs260/280 ≥1.8.
Size Range
Up to 25 kb
Yield
Up to 25 µg of plasmid DNA per preparation, depending on the plasmid copy number, culture growth
conditions, and strain of E. Coli utilized.
Supplemental Info
How to Increase Plasmid Yield
DOs and DON'Ts of Plasmid Purification