抗小鼠 CD194 (CCR4) 荧光标记PE 抗体

anti-mouse CD194 (CCR4) Antibody-PE详细如下：

Description ：Mouse CCR4 cDNA contains 1531 bp, and encodes a protein of 360 amino acids that is 85%identical to human CCR4. CCR4 binds CCL17 (TARG) and CCL22 (MDC). Naïve T cells,bearing receptors for cutaneous antigens, become activated in skin-draining lymph nodes andexpress cutaneous lymphocyte antigen (CLA), which confers to these cells the capacity tomigrate into the skin to exert their normal effector functions (1). CCR4 and CCR10 play animportant role in the ligand-mediated recruitment of T cells into the skin in mice and humans,specifically with regards to tethering, firm adhesion, and subsequent extravasation to the site ofinjury (2,3). CCR4 is expressed in cutaneous regulatory T cells (Tregs). These cells are crucialfor the induction and maintenance of self-tolerance and are present in peripheral tissues suchas skin and gut under normal, noninflamed conditions (4).In addition, recruitment of Foxp3+ Tregulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor andits ligand CCL22 (5).

Verified Reactivity ：Mouse

Antibody Type ：Monoclonal

Host Species ：Armenian Hamster

Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation ：The antibody was purified by affinity chromatography, and conjugated with PE under optimalconditions.

Concentration ：0.2 mg/m

Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.

Application ：FC - Quality tested

Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10 cells in100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.

Excitation Laser ：Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)