- ￥2500
- Vacara
- 10120
- 瑞典
- 2025年07月12日
- 小鼠
- Mouse, human
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北京博蕾德生物科技有限公司
- 库存：
1
- 靶点：
Collagen XI, CXI, collagen type XI, collagen type 11
- 克隆性：
单克隆
- 抗原来源：
Bovine Collagen XI (bCXI)
- 抗体英文名：
Mouse Anti-Collagen XI Antibody (L10D9)
- 抗体名：
Mouse Anti-Collagen XI Antibody (L10D9)
- 标记物：
无
- 宿主：
小鼠
- 适应物种：
Mouse, human
- 免疫原：
Bovine Collagen XI (bCXI)
- 亚型：
Mouse IgG2b, κ
- 形态：
液体
- 保存条件：
-20℃
- 浓度：
1 mg/ml
- 规格：
100ug/0.1ml
cat#10120
The antibody L10D9 can be used for immunoassay techniques such as ELISA1, immunohistochemistry (IHC)1, bead-based multiplex immunoassays1, Western blot1 and in vivo functional assays2.
Target with alternative names
Collagen XI, CXI, collagen type XI, collagen type 11
Immunogen
Bovine Collagen XI (bCXI)1
Species reactivity
Mouse, human
Isotype
Mouse IgG2b, κ
Cross-reactivity
Collagen type II (COL2A1)1
Concentration
1 mg/ml
Supplied in
PBS
Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at ‑20°C long term. Avoid repeated freeze/thaw cycles.
Recommended dilution
It is recommended the user determines the optimal dilution for their application. The typical starting working dilutions are: IHC 1:100, ELISA 1:200 - 1:500.
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文献和实验1. Tong D, Lönnblom E, Yau ACY, Nandakumar KS, Liang B, Ge C, Viljanen J, Li L, Bãlan M, Klareskog L, Chagin AS, Gjertsson I, Kihlberg J, Zhao M, Holmdahl R. A Shared Epitope of Collagen Type XI and Type II Is Recognized by Pathogenic Antibodies in Mice and Humans with Arthritis. Front Immunol. 2018 Apr 12;9:451. PMID: 29706949.
2. Li Y, Tong D, Liang P, Lönnblom E, Viljanen J, Xu B, Nandakumar KS, Holmdahl R. Cartilage-binding antibodies initiate joint inflammation and promote chronic erosive arthritis. Arthritis Res Ther. 2020 May 24;22(1):120. PMID: 32448385.
suspension. 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6) Add a pre-titrated
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
