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- ProSci
- 美国
- PSI-98-564-0.5mg
- 2025年07月15日
- WB
- 仓鼠
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37
- 保存条件：
Store vial at -20℃
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
仓鼠
- 应用范围：
WB
- 抗体英文名：
CD3e Antibody [145-2C11]
- 抗体名：
CD3e Antibody [145-2C11]
- 规格：
0.5mg
【干冰】CD3e 抗体 [145-2C11]，CD3e Antibody [145-2C11]，【干冰】CD3e 抗体 [145-2C11],CD3e Antibody [145-2C11]
产品名称：【干冰】CD3e 抗体 [145-2C11]-CD3e Antibody [145-2C11]
产品货号：PSI-98-564-0.5mg
产品规格：0.5mg
背景资料：CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The 145-2C11 monoclonal antibody recognizes an epitope on the 25 kDa epsilon chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble 145-2C11 can activate primed and naïve T cell in vitro. 145-2C11 can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL’s. Immobilized 145-2C11 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death.
保存建议：【干冰】CD3e 抗体 [145-2C11]-CD3e Antibody [145-2C11]Store vial at -20℃
应用类型：WB
点击：【干冰】CD3e 抗体 [145-2C11]-CD3e Antibody [145-2C11]查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验a 5-10 µg/ml solution of anti-CD3e (145-2C11) in sterile PBS. Calculate the number of wells required for each experimental condition and consider triplicate samples for each condition. For example, to coat one-half plate (48 wells) 2.6 ml of antibody
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
cell suspension 20 minutes to set up the assay 2-4 days incubation Method: ANTIBODY COATING OF THE ASSAY PLATE: 1) Prepare a 5-10 ug/ml solution of anti-CD3e (145-2C11
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