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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
WEE1 (phospho Ser642) antibody was raised against a peptide sequence around phosphorylation site of Serine 642 (S-V-S (p)-L-T) derived from Human WEE1.
- 保存条件:
Store antibody at -20℃ for up to one year
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
兔
- 应用范围:
WB
- 抗体英文名:
WEE1 (phospho Ser642) Antibody
- 抗体名:
WEE1 (phospho Ser642) Antibody
- 规格:
0.1mg
WEE1 (phospho Ser642) 抗体,WEE1 (phospho Ser642) Antibody,WEE1 (phospho Ser642) 抗体,WEE1 (phospho Ser642) Antibody
产品名称:WEE1 (phospho Ser642) 抗体-WEE1 (phospho Ser642) Antibody
产品货号:PSI-79-824-0.1mg
产品规格:0.1mg
背景资料:Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
保存建议:WEE1 (phospho Ser642) 抗体-WEE1 (phospho Ser642) AntibodyStore antibody at -20℃ for up to one year
应用类型:WB
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文献和实验【求助】于NF-KB 激活,P65磷酸化位点是ser536还是ser276?
伽利略 ............................... chenlydd 两者都有,蛋白磷酸化水平与其功能密切相关:检测其磷酸化水平可用抗磷酸化位点抗体检测。www.cellsignal.com公司有相应抗体出售。 P65磷酸化位点: regulates apoptosis: S536‑p regulates cell growth: S536‑p regulates cell
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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