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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
protected from prolonged exposure to light
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
Flow
- 抗体英文名:
CD45.2 Antibody [104] (PerCP-Cy5.5)
- 抗体名:
CD45.2 Antibody [104] (PerCP-Cy5.5)
- 规格:
0.1mg
CD45.2 抗体 [104] (PerCP-Cy5.5),CD45.2 Antibody [104] (PerCP-Cy5.5),CD45.2 抗体 [104] (PerCP-Cy5.5),CD45.2 Antibody [104] (PerCP-Cy5.5)
产品名称:CD45.2 抗体 [104] (PerCP-Cy5.5)-CD45.2 Antibody [104] (PerCP-Cy5.5)
产品货号:PSI-76-559-0.1mg
产品规格:0.1mg
背景资料:The 104 monoclonal antibody specifically reacts with the mouse CD45 molecule, the Leukocyte Common Antigen (LCA) which occurs in the alloantigen CD45.2-expressing mouse strains (C57BL/6, CBA, 129, A, AKR, C58, DBA/1, DBA/2, BALB/c, and C3H/He). The 104 monoclonal antibody does not react with the leukocytes of the CD45.1-expressing mouse strains (DA, SJL/J, RIII, and STS/A). The CD45 molecule is a member of the Protein Tyrosine Phosphatase (PTP) family, because its intracellular region contains two PTP domains. The extracellular region’s variability is caused by different levels of glycosylation, and the splicing of the 4, 5, and 6 exons. The isoforms found in the mouse strains depend on the activation state, maturation stage and cell type, and are very important in B and T lymphocytes antigen receptor signal transduction. The 104 antibody inhibits some of the B lymphocytes responses, reduces the serum IgG levels, and influences the autoimmune renal pathology.
保存建议:CD45.2 抗体 [104] (PerCP-Cy5.5)-CD45.2 Antibody [104] (PerCP-Cy5.5) protected from prolonged exposure to light
应用类型:Flow
点击:CD45.2 抗体 [104] (PerCP-Cy5.5)-CD45.2 Antibody [104] (PerCP-Cy5.5)查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验分泌。 随后进行表面染色:先以Fc阻断剂(2.4G2克隆,5 μg/mL)处理10分钟,再于4°C避光孵育15分钟,使用含1% BSA和0.1% NaN₃的DPBS缓冲液配制抗体,常用组合包括CD90.2、CD4、CD8、CD25、CD11b和CD45.2等标记。 固定步骤采用2%甲醛(FA,由含<3%甲醇的4% Roti-Histofix稀释),室温孵育40–60分钟。破膜与胞内染色使用0.1%皂苷缓冲液或1×Perm缓冲液,加入抗FoxP3、T-bet、RORγt、IL-17A或IFN-γ等抗体,4°C
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的主要靶标。2020 年 3 月 11 日,高福院士联合逯光文在 Cell Host & Microbe 发文:Structural Analysis of Rabies Virus Glycoprotein Reveals pH-Dependent Conformational Changes and Interactions with a Neutralizing Antibody。该研究报告了狂犬病毒 RABV-G 的晶体结构,分别在〜pH-8.0 时以游离形式和在〜pH-6.5 时与中和抗体
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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