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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr402 of rat p21 activated kinase 2 (PAK-2). The peptide sequence used is identical in PAK-1, 2 and 3. Note: Thr402 in PAK-2 corresponds to Thr423 in human PAK-1.
- 保存条件:
stored at -20℃ and is stable at -20℃ for at least 1 year
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
兔
- 应用范围:
WB
- 抗体英文名:
PAK1 (phospho Thr402) Antibody
- 抗体名:
PAK1 (phospho Thr402) Antibody
- 规格:
0.1mL
PAK1 (phospho Thr402) 抗体,PAK1 (phospho Thr402) Antibody,PAK1 (phospho Thr402) 抗体,PAK1 (phospho Thr402) Antibody
产品名称:PAK1 (phospho Thr402) 抗体-PAK1 (phospho Thr402) Antibody
产品货号:PSI-50-246-0.1mL
产品规格:0.1mL
背景资料:In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while -PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).
保存建议:PAK1 (phospho Thr402) 抗体-PAK1 (phospho Thr402) Antibody stored at -20℃ and is stable at -20℃ for at least 1 year
应用类型:WB
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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