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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A 10 amino acid peptide from the alpha smooth muscle isoform of actin was used as the immunogen for this Smooth Muscle Actin antibody.
- 保存条件:
Aliquot and Store at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
WB, Flow, IF, IHC
- 抗体英文名:
Smooth Muscle Actin Antibody [SPM332]
- 抗体名:
Smooth Muscle Actin Antibody [SPM332]
- 规格:
100ug
Smooth Muscle Actin 抗体 [SPM332],Smooth Muscle Actin Antibody [SPM332],Smooth Muscle Actin 抗体 [SPM332],Smooth Muscle Actin Antibody [SPM332]
产品名称:Smooth Muscle Actin 抗体 [SPM332]-Smooth Muscle Actin Antibody [SPM332]
产品货号:PSI-34-162-100ug
产品规格:100ug
背景资料:Actin is a major component of the cytoskeleton and is present in most cell types. This mAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
保存建议:Smooth Muscle Actin 抗体 [SPM332]-Smooth Muscle Actin Antibody [SPM332]Aliquot and Store at 2-8℃
应用类型:WB, Flow, IF, IHC
点击:Smooth Muscle Actin 抗体 [SPM332]-Smooth Muscle Actin Antibody [SPM332]查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
内参就是内部参照,一般是指管家基因编码表达的蛋白,它们在各个组织和细胞中的表达相对恒定,在检测蛋白的表达水平变化时常用它来做参照参。内参则可以校正蛋白质定量、上样过程中存在的误差,保证实验结果的准确性。 此外,内参可以作为空白对照,还可以检测蛋白转膜情况是否完全、整个 WB 显色发光体系是否正常。内参抗体种类很多,比如 β-Actin、β-Tubulin、GAPDH 等,下面简单介绍下如何选择内参。 一、目的蛋白分子量 选择内参抗体时,应该考虑目的蛋白分子量的大小。通常应该保证目的
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