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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A membrane preparation from human hepatocytes was used as the immunogen for the Macrophage / Histiocytoma Marker antibody.
- 保存条件:
Aliquot and Store at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
IHC-P
- 抗体英文名:
Macrophage / Histiocytoma Marker Antibody [D11]
- 抗体名:
Macrophage / Histiocytoma Marker Antibody [D11]
- 规格:
100ug
Macrophage / Histiocytoma Marker 抗体 [D11],Macrophage / Histiocytoma Marker Antibody [D11],Macrophage / Histiocytoma Marker 抗体 [D11],Macrophage / Histiocytoma Marker Antibody [D11]
产品名称:Macrophage / Histiocytoma Marker 抗体 [D11]-Macrophage / Histiocytoma Marker Antibody [D11]
产品货号:PSI-33-540-100ug
产品规格:100ug
背景资料:In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this mAb and a standard CD68 mAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This mAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This mAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.
保存建议:Macrophage / Histiocytoma Marker 抗体 [D11]-Macrophage / Histiocytoma Marker Antibody [D11]Aliquot and Store at 2-8℃
应用类型:IHC-P
点击:Macrophage / Histiocytoma Marker 抗体 [D11]-Macrophage / Histiocytoma Marker Antibody [D11]查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验静置约 1 h,直至凝胶溶液完全聚合凝固,小心拔出梳子,准备上样。 上样 向电泳槽中加入电泳缓冲液,要求两块玻璃内侧电泳液高于上样孔,外侧电泳槽内电泳液浸没凝胶底部,玻璃板内侧液面高于外侧,根据测得浓度计算的样本上样量点样(已处理好的样品,蛋白预染 Marker),保证每孔总蛋白量在 30-50 μg,确保总上样量体系小于 30 μL,注意上样时间尽量短,避免样品扩散。 电泳 上样后,盖好电泳槽的盖子,注意正负极,并选择适当的电压进行电泳。通常在不连续的系统中,上层浓缩胶的电泳电压(建议
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除了抗体原因,转膜恐怕是western blot成败的最关键因素。 园子里好多好多人在求助转膜时间,因此我们总结了一系列分子量蛋白转膜的时间,希望对大家有用。 蛋白来源:RAW264.7 总蛋白 蛋白名称(可保密):一些转录因子 蛋白分子量:40~70 KD WB用膜类型、孔径:0.45 NC 转膜方式(恒压、恒流):湿转 恒流400 mA 转膜时间:60~90 min PS.其实
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