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- ProSci
- 美国
- PSI-33-727-100ug
- 2025年07月15日
- WB, Flow, IF, IHC-P
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
- 保存条件：
Aliquot and Store at 2-8℃
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
小鼠
- 应用范围：
WB, Flow, IF, IHC-P
- 抗体英文名：
Villin Antibody [VIL1/1325]
- 抗体名：
Villin Antibody [VIL1/1325]
- 规格：
100ug
Villin 抗体 [VIL1/1325]，Villin Antibody [VIL1/1325]，Villin 抗体 [VIL1/1325],Villin Antibody [VIL1/1325]
产品名称：Villin 抗体 [VIL1/1325]-Villin Antibody [VIL1/1325]
产品货号：PSI-33-727-100ug
产品规格：100ug
背景资料：Villin (VIL1) is an epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. [UniProt]
保存建议：Villin 抗体 [VIL1/1325]-Villin Antibody [VIL1/1325]Aliquot and Store at 2-8℃
应用类型：WB, Flow, IF, IHC-P
点击：Villin 抗体 [VIL1/1325]-Villin Antibody [VIL1/1325]查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验Characterization and Validation of Cre‐Driver Mouse Lines
2. (A ) Schematic representation of the CreERT2 transgene used for pronuculei injection (Feil et al., ; Indra et al., ). The pair of primers used for RT‐qPCR amplification of Cre transcripts is located in the β‐globin region between a tissue‐specific promoter X (pX
-100透化15min。随后用4%牛血清白蛋白（BSA）封闭，依次加入一抗（靶蛋白）和二抗孵育（孵育时间和保存温度根据抗体类型调整）。用DAPI染细胞核，在显微镜下观察样本。计算靶蛋白阳性细胞数（N）与总细胞数（M）的比值，记为X=N/M。 ④标志物基因（实时荧光定量PCR）：使用商用RNeasy试剂盒或TRIzol法提取总RNA，然后用商用cDNA合成试剂盒进行逆转录。使用实时荧光定量PCR系统进行qPCR检测（实验组设置3个独立样本）。以商业化的人肠道（小肠或大肠）RNA作为阳性对照，GAPDH
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
Homo sapiens matrix metalloproteinase-like 1 (MMPL1), mRNA 695 NM_001950 Homo sapiens E2F transcription factor 4, p107/p130-binding (E2F4), mRNA 956 NM_003815 Homo sapiens a disintegrin and metalloproteinase domain 15 (metargidin) (ADAM15), mRNA
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