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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full length purified pig Insulin (aa 1-84) was used as the immunogen for this antibody.
- 保存条件:
Aliquot and Store at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
WB, IHC, IF
- 抗体英文名:
Insulin Antibody [2D11-H5 or INS05]
- 抗体名:
Insulin Antibody [2D11-H5 or INS05]
- 规格:
100ug
Insulin 抗体 [2D11-H5 or INS05],Insulin Antibody [2D11-H5 or INS05],Insulin 抗体 [2D11-H5 or INS05],Insulin Antibody [2D11-H5 or INS05]
产品名称:Insulin 抗体 [2D11-H5 or INS05]-Insulin Antibody [2D11-H5 or INS05]
产品货号:PSI-33-154-100ug
产品规格:100ug
背景资料:This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. antibody to insulin is an important beta-cell and insulinoma marker.
保存建议:Insulin 抗体 [2D11-H5 or INS05]-Insulin Antibody [2D11-H5 or INS05]Aliquot and Store at 2-8℃
应用类型:WB, IHC, IF
点击:Insulin 抗体 [2D11-H5 or INS05]-Insulin Antibody [2D11-H5 or INS05]查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
as a chimeric antibody by means of a mammalian expression vector. Proc.Casadei, J. et al. (1990).Natl. Acad. Sci. U. S. A. 87, 2047–2051. 13. Fully automated chemiluminescence immunoassay of insulin using antibody–protein A–bacterial magnetic particle complexes
】大鼠胰岛细胞原代培养 一周龄Wistar大鼠,断颈处死,于75%乙醇浸泡15分钟,无菌取出胰脏,于冰冷无菌D-Hanks液中漂洗,用眼科剪仔细清除脂肪、包膜、血管等胰外组织,转入青霉素小瓶中,加入少量无菌D-Hanks液,用眼科剪剪成0.1-1.0mm3大小的碎片,用滴管轻轻吸出上层细小脂肪碎块和油滴,再用无菌D-Hanks液反复清洗8~10次,加入10倍体积无菌消化酶液[胰蛋白酶-胶原酶消化液:0.05g胰蛋白酶(Sigma),0.025g Ⅴ型胶原酶(Sigma,663U/mg),0.05g
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