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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Intact CD47 purified from placenta was used as the immunogen for this antibody.
- 保存条件:
Aliquot and Store at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
WB, Flow, IF, Bl, IHC
- 抗体英文名:
CD47 Antibody [B6H12.2]
- 抗体名:
CD47 Antibody [B6H12.2]
- 规格:
100ug
CD47 抗体 [B6H12.2],CD47 Antibody [B6H12.2],CD47 抗体 [B6H12.2],CD47 Antibody [B6H12.2]
产品名称:CD47 抗体 [B6H12.2]-CD47 Antibody [B6H12.2]
产品货号:PSI-33-099-100ug
产品规格:100ug
背景资料:This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
保存建议:CD47 抗体 [B6H12.2]-CD47 Antibody [B6H12.2]Aliquot and Store at 2-8℃
应用类型:WB, Flow, IF, Bl, IHC
点击:CD47 抗体 [B6H12.2]-CD47 Antibody [B6H12.2]查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验to 1 liter with Qcheck to make sure pH is 3.0(2) Affigel Blue Running Buffer10 mM K2HPO4 2.28 g K2HPO40.15 M NaCl 8.2 g NaCl0.02% azide 0.2 g Na azideup to 1 liter with Q(3) 1.4 M NaCl8.1 g NaClup to 100 ml with Q(4) Saturated NH4SO4767 g NH4SO4add 1 liter Q
Antibody Staining of Ovaries 1. Dissect ovaries in EBR 2. Transfer ovaries into an Eppendorf tube and add 100 µl devitellinizing buffer and 600-µl heptane 3. Agitate gently for 10 minutes
with 6 ml Buffer B, 6 ml Buffer A, and 6 ml Buffer B. Wash with Storage Buffer and hold at 4°C. • To purify the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes. • Wash with 50 ml 10 mM Tris
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