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- ProSci
- 美国
- PSI-32-163-0.1mg
- 2025年07月14日
- E, WB, IF
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Ni-NTA purified truncated recombinant GSK3 alpha expressed in E. Coli strain BL21 (DE3).
- 保存条件：
stored at -20℃, stable for one year
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
小鼠
- 应用范围：
E, WB, IF
- 抗体英文名：
GSK3alpha Antibody [9D5G1]
- 抗体名：
GSK3alpha Antibody [9D5G1]
- 规格：
0.1mg
GSK3alpha 抗体 [9D5G1] ，GSK3alpha Antibody [9D5G1] ，GSK3alpha 抗体 [9D5G1] ,GSK3alpha Antibody [9D5G1]
产品名称：GSK3alpha 抗体 [9D5G1] -GSK3alpha Antibody [9D5G1]
产品货号：PSI-32-163-0.1mg
产品规格：0.1mg
背景资料：Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily; GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.
保存建议：GSK3alpha 抗体 [9D5G1] -GSK3alpha Antibody [9D5G1] stored at -20℃, stable for one year
应用类型：E, WB, IF
点击：GSK3alpha 抗体 [9D5G1] -GSK3alpha Antibody [9D5G1] 查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验【摘 要】目的：培养脑皮质微血管内皮细胞。方法：取1～5d Wistar乳鼠脑皮质,经不同孔径的筛网过滤后，用胶原酶振荡消化获得的微血管内皮细胞进行培养，用Ⅷ因子相关抗原免疫组化鉴定。结果：培养的细胞呈单层贴壁生长，7～9d呈典型的铺路卵石样征象。结论：建立了一种简便易行的培养脑皮质微血管内皮细胞的方法。【关键词】 细胞培养；微血管内皮细胞；Wistar大鼠脑微血管内皮细胞是构成血脑屏障的主要成分，具有特殊的形态结构和机能，在许多病理状态下起重要作用[1]。建立脑微血管内皮细胞体外培养，可获
Dual- and Triple-Color Fluorospot
in several publications and reagents/kits for various cytokine combinations and species are commercially available. The most common protocol for fluorospot includes the use of biotinylated detection antibodies for one cytokine and an FITC-labeled detection antibody
1.材料和方法1.1 DMEM/F12条件培养液（亚硒酸钠40μg·L-1，腐胺16.2 mg·L-1，转铁蛋白100 mg·L-1，胰岛素234U·L-1，甲状腺素0.4μg·L-1，黄体酮0.62 mg·L-1，谷氨酰胺3g·L-1）。DMEM/F12培养基、小牛血清购自Hyclone公司，兔抗鼠GC抗体、亚硒酸钠、腐胺等购自Sigma公司。1.2 视神经胶质细胞的来源、培养 取新生2d的Wistar大鼠10只（第三军医大学大坪医院野战外科研究所动物中心提供），无菌条件下取出双
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